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Catalog Number | orb1087553 |
---|---|
Category | Proteins |
Description | Human GDF-15, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ala 197 - Ile 308 (Accession # Q99988-1). |
Reactivity | Human |
Tag | N-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 38.8 kDa |
Target | GDF-15, MIC-1 |
UniProt ID | Q99988 |
Source | Human GDF-15, Fc Tag (orb1087553) is expressed from human 293 cells (HEK293). It contains AA Ala 197 - Ile 308 (Accession # Q99988-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human GFR alpha-like, His Tag at 5 μg/mL (100 μL/well) can bind Human GDF-15, Fc Tag with a linear range of 0.1-6 ng/mL (QC tested). |
Expression Region | Ala 197 - Ile 308 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_004855.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | GDF15,GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB,NRG-1 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 38.8 kDa. As a result of glycosylation, the protein migrates as 45-55 kDa under reducing (R) condition, and 70-90 kDa under non-reducing (NR) condition (SDS-PAGE). |
Expiration Date | 6 months from date of receipt. |
Immobilized Human GFR alpha-like, His Tag (Cat. No. orb545746) at 5 μg/mL (100 μL/well) can bind Human GDF-15, Fc Tag (Cat. No. orb1087553) with a linear range of 0.1-6 ng/mL (QC tested).
Biotin | |
90% | |
40.4 kDa | |
Biotinylated Human GDF-15, , Fc Tag (orb1184782) is expressed from human 293 cells (HEK293). It contains AA Ala 197 - Ile 308 (Accession # Q99988-1). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 38.4 kDa after removal of the signal peptide.The apparent molecular mass of hFc-GDF15 is approximately 35-55 kDa due to glycosylation. | |
Mammalian |