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Catalog Number | orb257495 |
---|---|
Category | Proteins |
Description | Human GAPDH, His Tag is expressed from E. coli cells. It contains AA Gly 2 - Glu 335 (Accession # AAH01601). |
Reactivity | Human |
Tag | N-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 36.9 kDa |
Target | GAPDH, G3PDH |
UniProt ID | P04406 |
Protein Sequence | AAH01601 |
Source | Human GAPDH, His Tag (orb257495) is expressed from E. coli cells. It contains AA Gly 2 - Glu 335 (Accession # AAH01601). |
Expression System | E. coli |
Biological Origin | Human |
Expression Region | Gly 2 - Glu 335 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH01601 |
Storage | -20℃ |
Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, pH7.5 |
Alternative names | GAPDH,GAPD,CDABP0047,OK,SW-cl.12 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.9 kDa. The protein migrates as 37-38 kDa under reducing (R) condition (SDS-PAGE). |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human GAPDH protein
> 85%, determined by SDS-PAGE | |
This protein contains the human GAPDH (P04406) (Met 1-Glu 335) was expressed with a polyhistidine tag at the N-terminus and expressed from E. coli. |
> 93%, determined by SDS-PAGE | |
This protein contains the human GAPDH (P04406) (Met 1-Glu 335) was expressed with a polyhistidine tag at the N-terminus and expressed from E. coli. |
98.00% | |
38 kDa (predicted); 38 kDa (reducing conditions) |