Human G-CSF R / CD114 Protein

• Catalog Number: orb257497
• Category: Proteins
• Description: Human G-CSF R, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Pro 621 (Accession # NP_000751.1).
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 67.4 kDa
• Target: G-CSF R
• UniProt ID: Q99062
• Source: Human G-CSF R, His Tag (orb257497) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Pro 621 (Accession # NP_000751.1).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human G-CSF, Tag Free at 2 μg/mL (100 μL/well) can bind Human G-CSF R, His Tag with a linear range of 0.01-0.313 μg/mL (QC tested).
• Expression Region: Glu 25 - Pro 621
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000751.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: CSF3R,CD114,GCSFR
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 67.4 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

References

Ko, Sung Hee et al. Nanofluidic device for continuous multiparameter quality assurance of biologics Nat Nanotechnol, 12, 804-812 (2017)

PubMed: 28530715

SDS-PAGE analysis of Human G-CSF R protein

Immobilized ActiveMax® Human G-CSF, Tag Free (Cat. No. orb257496) at 2 μg/mL (100 μL/well) can bind Human G-CSF R, His Tag (Cat. No. orb257497) with a linear range of 0.01-0.313 μg/mL (QC tested).