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Catalog Number | orb257477 |
---|---|
Category | Proteins |
Description | Human Flt-3 Ligand, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # P49771-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 19.9 kDa |
Target | Flt-3 Ligand |
UniProt ID | P49771 |
Source | Human Flt-3 Ligand, His Tag (orb257477) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # P49771-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human Flt-3, Fc Tag at 2 μg/mL (100 μL/well) can bind Human Flt-3 Ligand, His Tag with a linear range of 0.1-3 ng/mL (QC tested). |
Expression Region | Thr 27 - Pro 185 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001450.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | FLT3LG,FL,FLT3L,Flt3 ligand Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.9 kDa. The protein migrates as 28-30 kDa and 31-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Flt-3 Ligand protein
Immobilized Human Flt-3, Fc Tag (Cat. No. orb570275) at 2 μg/mL (100 μL/well) can bind Human Flt-3 Ligand, His Tag (Cat. No. orb257477) with a linear range of 0.1-3 ng/mL (QC tested).
Unconjugated | |
95% | |
18.0 kDa | |
Human Flt-3 Ligand, premium grade (orb257476) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # AAI26294). |
Unconjugated | |
90% | |
20.2 kDa | |
Mouse Flt-3 Ligand, His Tag (orb545725) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Arg 188 (Accession # P49772-1). |
Unconjugated | |
90% | |
19.9 kDa | |
Human Flt-3 Ligand, His Tag (orb511237) is expressed from Baculovirus-Insect cells. It contains AA Thr 27 - Pro 185 (Accession # P49771-1). |
Unconjugated | |
90% | |
19.6 kDa | |
Dog Flt-3 Ligand Protein, His Tag (orb1743027) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Arg 186 (Accession # A0A8C0M0A9). |
Unconjugated | |
90% | |
19.8 kDa | |
Rhinopithecus bieti Flt-3 Ligand Protein, His Tag (orb1743028) is expressed from human 293 cells (HEK293). It contains AA Gln 27 -Pro 184 (Accession # A0A2K6LTJ0). |