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Catalog Number | orb1516454 |
---|---|
Category | Proteins |
Description | Recombinant Human FGF21 Protein |
Tag | N-mFc-Avi |
Concentration | > 0.5 mg/ml |
Form/Appearance | Lyophilized powder |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. kDa |
Target | FGF21 |
UniProt ID | Q9NSA1 |
Protein Sequence | 29-209/209 |
Expression System | 3 |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | Fibroblast growth factor 21, FGF 21, FGF-21, FGFL, Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
> 96% as determined by SDS-PAGE and HPLC. | |
19.4 kDa | |
E.Coli |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.5 kDa after removal of the signal peptide. The apparent molecular mass of FGF21-hFc is approximately 40-55 kDa due to glycosylation. | |
Mammalian |
SDS-PAGE | |
Unconjugated | |
> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
HPLC, SDS-PAGE | |
Unconjugated | |
> 97% by SDS-PAGE and HPLC analyses | |
19.9 kDa |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
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