Human FGF basic protein

• Catalog Number: orb80022
• Category: Proteins
• Description: FGF 2 Human Recombinant (FGF-2) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton.The FGF-b is purified by proprietary chromatographic techniques.
• Tested applications: SDS-PAGE
• Purity: > 96.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: FGF 2
• Solubility (25°C): It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS.
• Biological Origin: E.coli
• Biological Activity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is < 0.5 ng/ml, corresponding to specific activity of 2,000,000 Units/mg.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The protein was lyophilized from concentrated (1mg/ml) solution in PBS, pH 7.4.
• Alternative names: Prostatropin protein, HBGH-2 protein, HBGF-2 prote
• Note: For research use only
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of Fibroblast Growth Factor-b as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human FGF basic protein