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| Catalog Number | orb257225 |
|---|---|
| Category | Proteins |
| Description | Human FGF basic, premium grade is expressed from E. coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4). |
| Reactivity | Human |
| Tag | Native |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 16.5 kDa |
| Target | FGF basic |
| UniProt ID | P09038 |
| Source | Human FGF basic, premium grade (orb257225) is expressed from E. coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4). |
| Expression System | E. coli |
| Biological Origin | Human |
| Biological Activity | Immobilized Human FGF basic, premium grade at 5 μg/mL (100 μL/well) can bind Human Glypican 3 Protein, Fc Tag with a linear range of 0.3-5 ng/mL (QC tested). |
| Expression Region | Pro 143 - Ser 288 |
| Endotoxins | 0.01 EU per μg |
| NCBI | AAI66646.1 |
| Storage | -20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | FGF2,BFGF,FGFB,FGF basic,HBGF-2 Read more... |
| Note | For research use only |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE). |
| Expiration Date | 6 months from date of receipt. |
Wu, Yu-Ting et al. Defining minimum essential factors to derive highly pure human endothelial cells from iPS/ES cells in an animal substance-free system Sci Rep, 5, 9718 (2015)
SDS-PAGE analysis of Human FGF basic protein
Immobilized Human FGF basic, premium grade (Cat. No. orb257225) at 5 μg/mL (100 μL/well) can bind Human Glypican 3 Protein, Fc Tag with a linear range of 0.3-5 ng/mL (QC tested).
- Item 1 of 2
Recombinant human FGFR1 Alpha (IIIc) protein, C-His-Avi (HEK293) [orb1516455]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result. kDa
50 μg - Item 1 of 1
Human FGFb (Q65I,C96S,N111G) Protein [orb1471749]
Greater than 95% as determined by reducing SDS-PAGE.
17.2 KDa
Mammalian
50 μg, 10 μg 
Human FGF2 protein [orb594729]
Greater than 95% as determined by SDS-PAGE.
17.4 kDa
E.coli
50 μg, 500 μg, 1 mg, 10 μgHuman FGF2 protein [orb594737]
Greater than 95% as determined by SDS-PAGE.
17.2 kDa
E.coli
1 mg, 500 μg, 10 μg, 50 μg
