Human FGF basic Protein

• Catalog Number: orb257225
• Category: Proteins
• Description: Human FGF basic, premium grade is expressed from E. coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4).
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 16.5 kDa
• Target: FGF basic
• UniProt ID: P09038
• Source: Human FGF basic, premium grade (orb257225) is expressed from E. coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4).
• Expression System: E. coli
• Biological Origin: Human
• Biological Activity: Immobilized Human FGF basic, premium grade at 5 μg/mL (100 μL/well) can bind Human Glypican 3 Protein, Fc Tag with a linear range of 0.3-5 ng/mL (QC tested).
• Expression Region: Pro 143 - Ser 288
• Endotoxins: 0.01 EU per μg
• NCBI: AAI66646.1
• Storage: -20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: FGF2,BFGF,FGFB,FGF basic,HBGF-2
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

References

Wu, Yu-Ting et al. Defining minimum essential factors to derive highly pure human endothelial cells from iPS/ES cells in an animal substance-free system Sci Rep, 5, 9718 (2015)

PubMed: 25864432

SDS-PAGE analysis of Human FGF basic protein

Immobilized Human FGF basic, premium grade (Cat. No. orb257225) at 5 μg/mL (100 μL/well) can bind Human Glypican 3 Protein, Fc Tag with a linear range of 0.3-5 ng/mL (QC tested).