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Catalog Number | orb80026 |
---|---|
Category | Proteins |
Description | FGF 9 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 207 amino acids and having a molecular mass of 23.4 kDa. The FGF-9 is purified by proprietary chromatographic techniques. |
Tested applications | SDS-PAGE |
Purity | > 95.0% as determined by RP-HPLC and SDS-PAGE. |
Conjugation | Unconjugated |
Target | FGF 9 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-9 Human Recombinant sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | APLGEVGNYF GVQDAVPFGN VPVLPVDSPV LLSDHLGQSE AGGLPRGPAVTDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIAVGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSNLYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELYKDILSQS. |
Biological Origin | E.coli |
Biological Activity | The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is < 0.5 ng/ml, corresponding to specific activity of 2,000,000 units/mg. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The sterile protein powder is lyophilized from 1mg/ml solution containing 1x PBS |
Alternative names | GAF (Glia-activating factor) protein, HBGF-9 prote Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human FGF 9 protein
> 95% as determined by SDS-PAGE and HPLC. | |
23.3 kDa | |
E.Coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
Unconjugated | |
Greater than 95% as determined by reducing SDS-PAGE. | |
23.44 KDa | |
E. Coli |
Greater than 95% as determined by SDS-PAGE. | |
23.44 kDa | |
E.coli |
> 95% by SDS-PAGE. | |
KMP1505, Recombinant Human FGF-9 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ser208) of human FGF-9 (Accession #P31371). |
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