You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb80028 |
---|---|
Category | Proteins |
Description | Fibroblast Growth Factor -21 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and an N-terminal Methionin (bold), having molecular weight of 19.7 kDa (calculated). The amino acid sequence of the recombinant human FGF21 is 100% homologous to the amino acid sequence of the human FGF21 without signal sequence.The FGF-21 is purified by proprietary chromatographic techniques. |
Tested applications | SDS-PAGE |
Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | FGF 21 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-21 Human Recombinant sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | MHPIPDS SPLLQFGGQV RQRYLYTDDA QQTEAHLEIR EDGTVGGAAD QSPESLLQLK ALKPGVIQIL GVKTSRFLCQ RPDGALYGSL HFDPEACSFR ELLLEDGYNV YQSEAHGLPL HLPGNKSPHRDPAPRGPARFLPLPGLPPAP PEPPGILAPQ PPDVGSSDPL SMVGPSQGRS PSYAS. |
Biological Origin | E.coli |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Filtered (0.4 um) and lyophilized from 0.5 mg/ml in 20 mM TRIS, 20 mM NaCl, pH 7.5. |
Alternative names | Fibroblast growth factor 21 protein, FGF-21 protei Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human FGF 21 protein
> 96% as determined by SDS-PAGE and HPLC. | |
19.4 kDa | |
E.Coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
HPLC, SDS-PAGE | |
Unconjugated | |
> 95 % by SDS-PAGE and HPLC analyses | |
19.7 kDa |
HPLC, SDS-PAGE | |
Unconjugated | |
> 97% by SDS-PAGE and HPLC analyses | |
19.9 kDa |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.5 kDa after removal of the signal peptide. The apparent molecular mass of FGF21-hFc is approximately 40-55 kDa due to glycosylation. | |
Mammalian |
Filter by Rating