Human Eotaxin protein

• Catalog Number: orb80123
• Category: Proteins
• Description: Eotaxin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8345.9 Dalton. The CCL11 is purified by proprietary chromatographic techniques.
• Tested applications: SDS-PAGE
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: Eotaxin
• Solubility (25°C): It is recommended to reconstitute the lyophilized Eotaxin Human Recombinant in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Ala-Ser-Val.
• Biological Origin: E.coli
• Biological Activity: The activity is determined by the chemoattract of human PBE (peripheral blood eosinophils) at concentration between 0.1-10 ng/ml.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The CCL11 was lyophilized from concentrated (1mg/ml) solution in water containing no additives.
• Alternative names: Small inducible cytokine A11 protein, CCL11 protei
• Note: For research use only
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.013 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of Eotaxin as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human Eotaxin protein