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Catalog Number | orb80016 |
---|---|
Category | Proteins |
Description | EMAP-II Recombinant Human produced in E.coli is a single, non-glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 18.3 kDa. The EMAP-II is purified by proprietary chromatographic techniques. |
Tested applications | SDS-PAGE |
Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | EMAP II |
Solubility (25°C) | It is recommended to reconstitute the lyophilized EMAP-II in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQM QNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK. |
Biological Origin | E.coli |
Biological Activity | Determined by the apoptotic effect on MCF-7 cells using concentration of 20-30 ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from concentrated (1mg/ml) solution in water containing 20 mM sodium Phosphate buffer pH=7.5 and 130mM sodium chloride. |
Alternative names | AIMP1 protein, EMAP2 protein, EMAP-2 protein, EMAP Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human EMAP II protein
> 98% as determined by SDS-PAGE and HPLC. | |
18.2 kDa | |
E.Coli |
Unconjugated | |
95% | |
35.2 kDa | |
Human AIMP1, His Tag (orb348795) is expressed from E. coli cells. It contains AA Ala 2 - Lys 312 (Accession # Q12904-1). |
Human | |
23.5 pg/mL-1500 pg/mL | |
5.8 pg/mL |