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Catalog Number | orb257415 |
---|---|
Category | Proteins |
Description | Human EGF R, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 70.5 kDa |
Target | EGF R |
UniProt ID | P00533 |
Protein Sequence | NP_005219.2 |
Source | Human EGF R, His Tag (orb257415) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Leu 25 - Ser 645 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_005219.2 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | EGFR,ERBB,ERBB1,HER1,PIG61,mENA Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.5 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Olsen, Dorte Aa et al. Quantification of EGFR autoantibodies in the amplification phenomenon of HER2 in breast cancer Clin Chem Lab Med, 51, 2325-2329 (2013)
SDS-PAGE analysis of Human EGF R protein
Line graph illustrates about the sensitivity range of Human EGF R protein using different concentrations
> 97% as determined by SDS-PAGE and HPLC. | |
9.7 kDa | |
E.Coli |
Human | |
0.312 ng/mL-20 ng/mL | |
0.019 ng/mL |
Human | |
0.16-10ng/mL | |
0.1 ng/mL |
Unconjugated | |
95% | |
94.7 kDa | |
Human EGF R, Fc Tag (orb257416) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1). |
Unconjugated | |
95% | |
71.2 kDa | |
Mouse EGF R, His Tag (orb257420) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 647 (Accession # Q01279-1). |
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