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Catalog Number | orb391653 |
---|---|
Category | Proteins |
Description | Recombinant human EGF protein is produced by our E. coli expression system and the target gene encoding Asn971-Arg1023 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 200mM NaCl, pH 7.0. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | EGF |
Entrez | 1950 |
UniProt ID | P01133 |
Protein Sequence | NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 200mM NaCl, pH 7.0. |
Alternative names | Pro-epidermal growth factor; EGF Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
8.3 kDa | |
Human EGF Protein, His Tag, premium grade (orb762429) is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1). |
Unconjugated | |
95% | |
70.5 kDa | |
Human EGF R, His Tag (orb257415) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1). |
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