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Catalog Number | orb1088168 |
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Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynamin 1 Like Protein(DNM1L). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dynamin 1 Like Protein(DNM1L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynamin 1 Like Protein(DNM1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dynamin 1 Like Protein(DNM1L) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Human |
Range | 0.16-10 ng/mL |
UniProt ID | O00429 |
Sensitivity | 0.067 ng/mL |
Protein Length | 3.5h |
Alternative names | DRP1, DLP1, DVLP, DYMPLE, HDYNIV, VPS1, Dynamin-re Read more... |
Note | For research use only |
Sample Types | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
Human | |
0.156-10ng/ml | |
0.094ng/ml |
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