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Catalog Number | orb334894 |
---|---|
Category | Proteins |
Description | Human Dkk-1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 52.4 kDa |
Target | Dkk-1 |
UniProt ID | O94907 |
Protein Sequence | NP_036374.1 |
Source | Human Dkk-1, Fc Tag (orb334894) is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Thr 32 - His 266 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_036374.1 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | DKK1,SK Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.4 kDa. The protein migrates as 67-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Dkk-1 protein
Unconjugated | |
95% | |
26.6 kDa | |
Human Dkk-1 Protein, His Tag, premium grade (orb257395) is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1). |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 51.9 kDa after removal of the signal peptide.The apparent molecular mass of DKK1-hFC is approximately 70 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 52.0 kDa after removal of the signal peptide. The apparent molecular mass of DKK1-mFc is approximately 55-70 kDa due to glycosylation. | |
E.coli |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 26.6 kDa after removal of the signal peptide. The apparent molecular mass of DKK1-His is approximately 35-55 kDa due to glycosylation. | |
E.coli |