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| Catalog Number | orb257395 |
|---|---|
| Category | Proteins |
| Description | Human Dkk-1 Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1). |
| Reactivity | Human |
| Tag | C-6×His |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 26.6 kDa |
| Target | Dkk-1 |
| UniProt ID | O94907 |
| Source | Human Dkk-1 Protein, His Tag, premium grade (orb257395) is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Immobilized Human Dkk-1 Protein, His Tag, premium grade at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag with a linear range of 0.01-0.156 μg/mL (QC tested). |
| Expression Region | Thr 32 - His 266 |
| Endotoxins | 0.1 EU per μg |
| NCBI | NP_036374.1 |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | DKK1,SK Read more... |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
Li, Chunyan et al. The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling Biomed Res Int, 2015, 345413 (2015)
SDS-PAGE analysis of Human Dkk-1 protein
Immobilized Human Dkk-1 Protein, His Tag, premium grade (Cat. No. orb257395) at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. orb570288) with a linear range of 0.01-0.156 μg/mL (QC tested).
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Human DKK1 Protein, hFc Tag [orb1173930]
Unconjugated
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 51.9 kDa after removal of the signal peptide.The apparent molecular mass of DKK1-hFC is approximately 70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgHuman DKK1 protein [orb391620]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μgHuman DKK1 ELISA Kit [orb405710]
Human
62.5 pg/mL-4000pg/mL
15.6 pg/mL
10 x 96 t, 5 x 96 t, 96 t, 48 t, 24THuman DKK1 Protein, mFc Tag [orb1743312]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 52.0 kDa after removal of the signal peptide. The apparent molecular mass of DKK1-mFc is approximately 55-70 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg
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