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Catalog Number | orb348817 |
---|---|
Category | Proteins |
Description | Human CX3CL1, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Gln 341 (Accession # AAH01163). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 35.5 kDa |
Target | CX3CL1, Fractalkine |
UniProt ID | P78423 |
Source | Human CX3CL1, His Tag (orb348817) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Gln 341 (Accession # AAH01163). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Gln 25 - Gln 341 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH01163 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | CX3CL1,Fractalkine Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.5 kDa. The protein migrates as 50-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human CX3CL1 protein
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 34.4 kDa after removal of the signal peptide. | |
Mammalian |
Unconjugated | |
90% | |
36.5 kDa | |
Cynomolgus CX3CL1 Protein, His Tag (orb1496211) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 352 (Accession # A0A872TN34-1). |
Greater than 90.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
98.00% | |
35 kDa (predicted); 50-70 kDa (reducing condition, due to glycosylation) |
> 95% by SDS-PAGE. | |
Recombinant Human CX3CL1/Fractalkine Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Gln25-Arg339) of human CX3CL1/Fractalkine (Accession #P78423) fused with a 6��His Tag at the C-terminus. |