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Catalog Number | orb753123 |
---|---|
Category | Proteins |
Description | CST3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 141 amino acids (27-146 a.a.) and having a molecular mass of 15.6kDa.CST3 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques. |
Form/Appearance | Sterile Filtered colorless solution. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MSSPGKPPRL VGGPMDASVE EEGVRRALDF AVGEYNKASN DMYHSRALQV VRARKQIVAG VNYFLDVELG RTTCTKTQPN LDNCPFHDQP HLKRKAFCSF QIYAVPWQGT MTLSKSTCQD A |
Source | E.coli |
Biological Activity | The IC50 value is < 2.0nM. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25˚C. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | The CST3 solution (0.25mg/1ml) contains 0.1M NaCl, 20mM Tris-HCl buffer (pH 8.0) and 20% glycerol. |
Alternative names | Cystatin-C, Cystatin-3, Neuroendocrine basic polyp Read more... |
Note | For research use only |
Application notes | Recombinant & Natural Proteins |
Expiration Date | 6 months from date of receipt. |
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
> 95% as determined by SDS-PAGE | |
15 kDa |