You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb257232 |
---|---|
Category | Proteins |
Description | Human Complement C5a, Tag Free is expressed from E. coli cells. It contains AA Leu 679 - Arg 751 (Accession # P01031). |
Reactivity | Human |
Tag | Native |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 8.3 kDa |
Target | Complement C5a |
UniProt ID | P01031 |
Source | Human Complement C5a, Tag Free (orb257232) is expressed from E. coli cells. It contains AA Leu 679 - Arg 751 (Accession # P01031). |
Expression System | E. coli |
Biological Origin | Human |
Biological Activity | Immobilized Human Complement C5a, Tag Free at 2 μg/mL (100 μL/well) can bind Anti-C5a MAb, Human IgG1 with a linear range of 0.8-13 ng/mL (QC tested). |
Expression Region | Leu 679 - Arg 751 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | C5a,Complement Component 5a Read more... |
Note | For research use only |
Application notes | This protein carries no "tag". The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE). |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human C5a protein
Immobilized Human Complement C5a, Tag Free (Cat. No. orb257232) at 2 μg/mL (100 μL/well) can bind Anti-C5a MAb, Human IgG1 with a linear range of 0.8-13 ng/mL (QC tested).
Unconjugated | |
90% | |
34.6 kDa | |
Human Complement C5a, Fc Tag (orb612128) is expressed from human 293 cells (HEK293). It contains AA Leu 679 - Arg 751 (Accession # P01031-1). |
Unconjugated | |
95% | |
10.2 kDa | |
Human Complement C5a, His Tag (orb1496182) is expressed from E. coli cells. It contains AA Leu 679 - Arg 751 (Accession # P01031-1). |
Greater than 85% as determined by SDS-PAGE. | |
38.9 kDa | |
in vitro E.coli expression system |
Greater than 90% as determined by SDS-PAGE. | |
12.3 kDa | |
Mammalian cell |