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Catalog Number | orb1087565 |
---|---|
Category | Proteins |
Description | Human Complement C3, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Asn 1663 (Accession # P01024-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 186.8 kDa |
Target | Complement C3 |
UniProt ID | P01024 |
Source | Human Complement C3, His Tag (orb1087565) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Asn 1663 (Accession # P01024-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human Complement C3, His Tag at 1 μg/mL (100 μL/well) can bind Human Anti-C3,Human IgG1 Human Kappa with a linear range of 0.1-4 ng/mL (QC tested). |
Expression Region | Ser 23 - Asn 1663 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000055.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | Complement C3,CPAMD1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 186.8 kDa. The protein migrates as 70 kDa, 100-120 kDa and 160-180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Human Complement C3, His Tag (Cat. No. orb1087565) at 1 μg/mL (100 μL/well) can bind Human Anti-C3, Human IgG1 Human Kappa with a linear range of 0.1-4 ng/mL (QC tested).
Unconjugated | |
95% | |
71.3 kDa (β chain) and 113.0 kDa (α chain) | |
Cynomolgus Complement C3, His Tag (orb1101181) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Asn 1663 (Accession # G7PYU9-1). |
Biotin | |
95% | |
188.5 kDa | |
Biotinylated Human Complement C3, His, (orb1176546) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Asn 1663 (Accession # P01024-1). |
Unconjugated | |
90% | |
186.1 kDa | |
Mouse Complement C3, His Tag (orb1101180) is expressed from human 293 cells (HEK293). It contains AA Ile 25 - Asn 1663 (Accession # P01027-1). |
Human | |
62.5 pg/ml - 4000 pg/ml | |
30 pg/ml |
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