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Catalog Number | orb668876 |
---|---|
Category | Proteins |
Description | Human Coagulation factor XI, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Val 625 (Accession # P03951-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 69.9 kDa |
Target | Coagulation factor XI, FXI |
UniProt ID | P03951 |
Source | Human Coagulation factor XI, His Tag (orb668876) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Val 625 (Accession # P03951-1). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Glu 19 - Val 625 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | 25 mM HEPES, 150 mM NaCl, pH7.5 |
Alternative names | Coagulation factor XI,FXI,PTA,F11 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The proprotein is activated by factor XIIa (or XII) and cleaved after Arg-387 into the light chain with calculated MW of 28.7 kDa, which contains the active site, and the heavy chain with calculated MW of 41.2 kDa. The protein migrates as 47 kDa (heavy chain) and 35 kDa (light chain) under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
> 85%, determined by SDS-PAGE | |
This protein contains the human F11 (NP_000119.1) precursor (Met 1-Val 625) with a carboxy-terminal polyhistidine tag was expressed from HEK293 Cells. |