Human CLDN18.2 protein

• Catalog Number: orb865262
• Category: Proteins
• Description: Recombinant Human Claudin-18.2(CLDN18.2)-VLPs (Active)
• Tag: C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
• Form/Appearance: Lyophilized powder
• Purity: Testing in progress.
• MW: 29.1 kDa
• UniProt ID: P56856
• Protein Sequence: MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV
• Protein Length: Full Length of Isoform A2
• Source: Mammalian cell
• Biological Origin: Homo sapiens (Human)
• Expression Region: 1-261aa
• Endotoxins: Less than 1.0 EU/ug as determined by LAL method.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
• Buffer/Preservatives: Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
• Alternative names: CLDN18.2
• Note: For research use only
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
• Expiration Date: 6 months from date of receipt.

Measured by its binding ability in a functional ELISA. Immobilized human CLDN18.2 at 5 μg/ml can bind anti-CLDN18.2 recombinant Monoclonal Antibody, the the EC50 is 5.225-9.256 ng/ml.

WB analysis of the three bands respectively correspond to monomer, Homodimer, Homotrimer using Human CLDN18.2 protein

Western blot analysis of the two bands respectively correspond to monomer, Homodimer using Human CLDN18.2 protein