Human CLDN18.2 full length protein-VLP

• Catalog Number: orb1291767
• Category: Proteins
• Description: Human Full length Claudin 18.2 Protein-VLP
• Reactivity: Human
• Tag: C-Flag Tag
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• MW: The Human Claudin 18.2 Protein has a predicted MW of 29 kDa.Due to PTM, the actual MW on SDS-PAGE gel is around 50kDa.
• Target: CLDN18.2
• UniProt ID: P56856
• Source: Mammalian
• Expression System: HEK293
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Alternative names: Claudin 18.2
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

ELISA plates were pre-coated with 0.5ug/per well purified human CLDN18.2 VLP. Serial diluted Anti-CLDN18.2 monoclonal antibody (Zolbetuximab biosililar; IMAB362) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for IMAB362 binding with CLDN18.2 VLP is 15.37 ng/ml.

FACS analysis of CLDN18.2 VLP.A. Negative Control 1: CLDN18.2 VLP were stained only with Goat anti-human IgG Fc-PE secondary antibody.B. Negative Control 2: Control VLP were stained with anti-CLDN18.2 antibody (Zolbetuximab biosimilar; IMAB362) at 1 μg/mL, followed by Goat anti-human IgG Fc-PE secondary antibody.C. Negative Control 3: CLDN18.2 VLP were stained with anti-BCMA antibody (an irrelevant antibody) at 1 μg/mL, followed by Goat anti-human IgG Fc-PE secondary antibody.D. CLDN18.2 VLP were stained with anti-CLDN18.2 antibody (Zolbetuximab biosimilar; IMAB362) at 1 μg/mL, followed by Goat anti-human IgG Fc-PE secondary antibody.