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Catalog Number | orb759147 |
---|---|
Category | Proteins |
Description | Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a Fc tag at the C-terminus. |
Tag | C-Fc |
Form/Appearance | Supplied as a 0.2 um filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5. |
UniProt ID | AAH65937.1 |
Expression System | Human Cells |
Expression Region | Trp27-Lys547 |
Endotoxins | Less than 0.1 ng/ug (1 EU/ug) |
Storage | Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles. |
Alternative names | 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73 Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Functional Studies, IA, WB | |
> 95% | |
58.8 | |
HEK293 cells |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 84.1 kDa after removal of the signal peptide.The apparent molecular mass of CD73-hFc is approximately 55-100 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
Greater than 95% as determined by SDS-PAGE. | |
58.76 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
84.6 kDa | |
E.coli |
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