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Catalog Number | orb753100 |
---|---|
Category | Tools |
Description | CD72 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 485 amino acids (117-359a.a.) and having a molecular mass of 55.3kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).CD72 is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques. |
Form/Appearance | Sterile Filtered colorless solution. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Protein Sequence | ADPRYLQVSQ QLQQTNRVLE VTNSSLRQQL RLKITQLGQS AEDLQGSRRE LAQSQEALQVEQRAHQAAEG QLQACQADRQ KTKETLQSEE QQRRALEQKL SNMENRLKPF FTCGSADTCCPSGWIMHQKS CFYISLTSKN WQESQKQCET LSSKLATFSE IYPQSHSYYF LNSLLPNGGSGNSYWTGLSS NKDWKLTDDT QRTRTYAQSS KCNKVHKTWS WWTLESESCR SSLPYICEMTAFRFPDLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSHEDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKALPAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPENNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH |
Source | Sf9, Baculovirus cells |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | CD72 protein solution (0.5mg/ml) contains 50mM Tris-HCl buffer (pH6.8), 0.2M NaCl, 2mM DTT and 50% glycerol. |
Alternative names | CD72, CD72b, LYB2, B-cell differentiation antigen Read more... |
Note | For research use only |
Application notes | Recombinant & Natural Proteins |
Expiration Date | 12 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 54.2 kDa after removal of the signal peptide. The apparent molecular mass of hFc-CD72 is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
Unconjugated | |
95% | |
54.2 kDa | |
Human CD72, Fc Tag (orb257261) is expressed from human 293 cells (HEK293). It contains AA Arg 117 - Asp 359 (Accession # AAH30227). |
≥90% as determined by SDS-PAGE | |
This protein contains the human CD72(Arg117-Asp359) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
> 90% by SDS-PAGE. | |
KMP1480, Recombinant Human CD72 Protein is produced by E.coli expression system. The target protein is expressed with sequence (Arg117-Cys226) of human CD72 (Accession #P21854) fused with a Trx, 6xHis Tag at the N-terminus. |
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