Human CD4 Mouse Monoclonal Antibody

• Catalog Number: orb526621
• Category: Antibodies
• Description: Human CD4 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HIT4a
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: KLH conjugated synthetic peptide derived from human CD4
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: CD4
• UniProt ID: P01730
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4).
• Alternative names: LHR; BA-1; CD 44; CD44 antigen; CD44 molecule; CD4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): U937. Primary Antibody (green line): Mouse Anti-CD4 antibody (orb526621), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.