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    Human CD3E protein

    Human CD3E protein

    Catalog Number: orb706889

    DispatchUsually dispatched within 5-10 working days
    $ 3,194.00
    Catalog Numberorb706889
    CategoryProteins
    DescriptionRecombinant Human CD3e Protein is produced by mammalian expression system and the target gene encoding Asp23-Asp126 is expressed with a Fc tag at the C-terminus.
    Tested applicationsELISA, MS, SDS-PAGE, WB
    ReactivityHuman
    Form/AppearanceLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
    PurityGreater than 95% as determined by reducing SDS-PAGE.
    TargetCD3E
    Entrez916
    UniProt IDP07766
    Protein SequenceDGNEEMGGIT QTPYKVSISG TTVILTCPQY PGSEILWQHN DKNIGGDEDD KNIGSDEDHL SLKEFSELEQ SGYYVCYPRG SKPEDANFYL YLRARVCENC MEMDDIEGRM DEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS REEMTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK
    SourceHuman cells
    EndotoxinsEndotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
    StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
    Buffer/PreservativesLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
    Alternative namesT3E protein, T-cell surface glycoprotein CD3 epsil
    Read more...
    NoteFor research use only
    Application notesAlways centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Expiration Date6 months from date of receipt.
    • Human CD3 epsilon Protein [orb348813]

      Unconjugated

      95%

      39.2 kDa (CD3E) & 38.6 kDa (CD3G)

      Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (orb348813) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1 (CD3E) & P09693-1 (CD3G)).

      50 μg, 1 mg
    • Human CD3 epsilon Protein [orb257326]

      Unconjugated

      90%

      16.9 kDa

      Human CD3 epsilon, His Tag (orb257326) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).

      1 mg, 100 μg
    • Cynomolgus CD3 epsilon Protein [orb257323]

      Unconjugated

      90%

      16.1 kDa

      Cynomolgus CD3 epsilon, His Tag (orb257323) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5-1).

      1 mg, 100 μg
    • Human CD3E protein [orb391409]

      ELISA,  MS,  SDS-PAGE,  WB

      Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

      Human cells

      500 μg, 50 μg, 10 μg
    • Human CD3D Protein, His Tag and Human CD3E Protein, hFc Tag [orb1743385]

      Unconjugated

      The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

      The protein has a predicted molecular mass of 10.4 and 37.9 kDa after removal of the signal peptide. The apparent molecular mass of CD3D-His and CD3E-hFc is approximately 35-55 kDa due to glycosylation.

      Mammalian

      10 μg, 50 μg, 100 μg
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