Human CD3 epsilon Protein

• Catalog Number: orb257326
• Category: Proteins
• Description: Human CD3 epsilon, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 16.9 kDa
• Target: CD3 epsilon
• UniProt ID: P07766
• Protein Sequence: NP_000724.1
• Source: Human CD3 epsilon, His Tag (orb257326) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).
• Expression System: HEK293
• Biological Origin: Human
• Expression Region: Asp 23 - Asp 126
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000724.1
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: FLJ18683,T3E,TCRE,CD3E,CD3-epsilon
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.9 kDa. Maybe caused by unknown PTMs, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human CD3 epsilon protein

Immobilized Anti Human CD3 MAb, mouse IgG1 at 2 ug/mL (100 uL/well) can bind Human CD3E, His Tag with a linear range of 0.4-6.25 ng/mL.