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Catalog Number | orb706867 |
---|---|
Category | Proteins |
Description | Recombinant Human CD274 Protein is produced by mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a Flag tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB 150mM Nacl pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CD274 |
Entrez | 29126 |
UniProt ID | Q9NZQ7 |
Protein Sequence | FTVTVPKDLY VVEYGSNMTI ECKFPVEKQL DLAALIVYWE MEDKNIIQFV HGEEDLKVQH SSYRQRARLL KDQLSLGNAA LQITDVKLQD AGVYRCMISY GGADYKRITV KVNAPYNKIN QRILVVDPVT SEHELTCQAE GYPKAEVIWT SSDHQVLSGK TTTTNSKREE KLFNVTSTLR INTTTNEIFY CTFRRLDPEE NHTAELVIPE LPLAHPPNER TDYKDDDDK |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20mM PB 150mM Nacl pH 7.4. |
Alternative names | B7H1 protein, PDCD1L1 protein, PDCD1LG1 protein, P Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
51.3 kDa | |
Human PD-L1, Fc Tag (orb257754) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1). |
Unconjugated | |
98% | |
26.0 kDa | |
Human PD-L1, His Tag (orb257752) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1 ). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
95% | |
51.4 kDa | |
Mouse PD-L1, Fc Tag (orb257764) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693). |
Unconjugated | |
95% | |
27.1 kDa | |
Cynomolgus / Rhesus macaque PD-L1, His Tag (orb257750) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # G7PSE7-1). In the region Phe 19 - Arg 238, the AA sequence of Cynomolgus and Rhesus macaque PD-L1 are homologus. |
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