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Catalog Number | orb257270 |
---|---|
Category | Proteins |
Description | Human CD14, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Met 344 (Accession # AAH10507). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 98% |
Conjugation | Unconjugated |
MW | 35.9 kDa |
Target | CD14 |
UniProt ID | P08571 |
Protein Sequence | AAH10507 |
Source | Human CD14, His Tag (orb257270) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Met 344 (Accession # AAH10507). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Thr 20 - Met 344 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH10507 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | CD14 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.9 kDa. The protein migrates as 40-53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human CD14 protein
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 36.7 kDa after removal of the signal peptide. The apparent molecular mass of CD14(20-352)-7xHis is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 35.9 kDa after removal of the signal peptide. The apparent molecular mass of CD14(20-344)-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
95% | |
62.2 kDa | |
Human CD14, Fc Tag (orb257273) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Cys 352 (Accession # P08571-1). |
Greater than 90% as determined by SDS-PAGE. | |
62.2 kDa | |
E.coli |
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