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Catalog Number | orb1176553 |
---|---|
Category | Proteins |
Description | Human CD131, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Trp 17 - Trp 443 (Accession # P32927-1). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 75.1 kDa |
Target | IL-3 R Beta, CD131 |
UniProt ID | P32927 |
Source | Human CD131, Fc Tag (orb1176553) is expressed from human 293 cells (HEK293). It contains AA Trp 17 - Trp 443 (Accession # P32927-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Human CD131, Fc Tag on Protein A Biosensor, can bind Human GM-CSF, premium grade with an affinity constant of 7.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Trp 17 - Trp 443 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000386.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 |
Alternative names | IL-3 R Beta,CD131,Common beta Chain Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 75.1 kDa. The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Loaded Human CD131, Fc Tag (Cat. No. orb1176553) on Protein A Biosensor, can bind Human GM-CSF, premium grade (Cat. No. orb257510) with an affinity constant of 7.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).