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Catalog Number | orb865263 |
---|---|
Category | Proteins |
Description | Recombinant Human C-C chemokine receptor type 8(CCR8)-VLPs (Active) |
Tag | C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions) |
Form/Appearance | Lyophilized powder |
MW | 42.2 kDa |
UniProt ID | P51685 |
Protein Sequence | MDYTLDLSVTTVTDYYYPDIFSSPCDAELIQTNGKLLLAVFYCLLFVFSLLGNSLVILVLVVCKKLRSITDVYLLNLALSDLLFVFSFPFQTYYLLDQWVFGTVMCKVVSGFYYIGFYSSMFFITLMSVDRYLAVVHAVYALKVRTIRMGTTLCLAVWLTAIMATIPLLVFYQVASEDGVLQCYSFYNQQTLKWKIFTNFKMNILGLLIPFTIFMFCYIKILHQLKRCQNHNKTKAIRLVLIVVIASLLFWVPFNVVLFLTSLHSMHILDGCSISQQLTYATHVTEIISFTHCCVNPVIYAFVGEKFKKHLSEIFQKSCSQIFNYLGRQMPRESCEKSSSCQQHSSRSSSVDYIL |
Protein Length | Full Length |
Source | Mammalian cell |
Biological Origin | Homo sapiens (Human) |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized human CCR8 at 5 μg/ml can bind Anti-CCR8 recombinant Antibody, the the EC50 is 11.13-17.29 ng/ml. |
Expression Region | 1-355aa |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4 |
Alternative names | (CC chemokine receptor CHEMR1) (CMKBRL2) (Chemokin Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing. |
Expiration Date | 6 months from date of receipt. |
Detected by Mouse anti-6*His monoclonal antibody.
Detected by Anti-CCR8 recombinant Antibody
Measured by its binding ability in a functional ELISA. Immobilized human CCR8 at 5 μg/ml can bind Anti-CCR8 recombinant Antibody, the the EC50 is 11.13-17.29 ng/ml.
The presence of VLP-like structures was confirmed by TEM
Loaded Anti-Human CCR8 Antibody on 96-Flat plate, can bind Human CCR8, with an affinity constant of <1 pM as determined in BLI assay (Gator Prime).
The purity of VLPs was greater than 90% as determined by SEC-HPLC
The human full length CCR8 Protein has a MW of 40.7 kDa | |
HEK293 |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 30.0 kDa after removal of the signal peptide. The apparent molecular mass of mCCR8-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
The human full length CCR8 Protein has a MW of 40.7 kDa | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
7.7 kDa | |
Yeast |