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Catalog Number | orb257382 |
---|---|
Category | Proteins |
Description | Human Cathepsin B, His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 18 - Ile 339 (Accession # P07858-1 (L26V)). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 36.7 kDa & 29.5 kDa |
Target | Cathepsin B |
UniProt ID | P07858 |
Protein Sequence | NP_001899.1 |
Source | Human Cathepsin B, His Tag (orb257382) is expressed from human 293 cells (HEK293). It contains AA Arg 18 - Ile 339 (Accession # P07858-1 (L26V)). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Arg 18 - Ile 339 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001899.1 |
Storage | -20℃ |
Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, pH8.0 |
Alternative names | CTSB,CPSB,APPS Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The Human Cathepsin B will be further processed into mature form (Leu 80-Ile 339). The protein has a calculated MW of 36.7 kDa (pro-form) and 29.5 kDa (mature-form). The protein migrates as 42-50 kDa and 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Zilbermintz, Leeor et al. Identification of agents effective against multiple toxins and viruses by host-oriented cell targeting Sci Rep, 5, 13476 (2015)
SDS-PAGE analysis of Human Cathepsin B protein
≥90% as determined by SDS-PAGE | |
This protein contains the human CTSB(Arg18-Ile339) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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