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Catalog Number | orb424506 |
---|---|
Category | Tools |
Description | Recombinant of human Carbonic Anhydrase 2 protein |
Form/Appearance | Sterile Filtered colorless solution. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK |
Source | Escherichia Coli |
Biological Activity | Specific activity is 50-70 nmoles/min/µg and was obtained by measuring the increase in the amount of p-nitrophenol by its esterase activity. Specific activity is defined as the amount of p-nitrophenol that 1ug of enzyme can reduce at 25C for 1 minute. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | Carbonic Anhydrase 2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50mM NaCl and 10% glycerol. |
Alternative names | Carbonic anhydrase 2, Carbonate dehydratase 2, Car Read more... |
Note | For research use only |
Application notes | Enzymes |
Expiration Date | 12 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Human | |
0.312 ng/mL-20 ng/mL | |
0.078 ng/mL |
Greater than 90% as determined by SDS-PAGE. | |
45.1 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human CA11(His24-Arg328) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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