Human Betacellulin protein

• Catalog Number: orb80011
• Category: Proteins
• Description: Betacellulin Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9 kDa. Betacellulin Human Recombinant is purified by proprietary chromatographic techniques.
• Tested applications: SDS-PAGE
• Purity: > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: BTC
• Solubility (25°C): It is recommended to reconstitute the lyophilized BTC Human in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Gly-Asn-Ser-Thr.
• Biological Origin: E.coli
• Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is 0.05 ng/ml. corresponding to Specific Activity of > 2.0 x 10,000,000 IU/mg.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The Betacellulin Human Recombinant was lyophilized after extensive dialysis against 20 mM phosphate buffer pH-7.4.
• Note: For research use only
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of BTC as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human Betacellulin protein