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Catalog Number | orb594732 |
---|---|
Category | Proteins |
Description | Recombinant Human Brain-derived neurotrophic factor(BDNF) (Active) |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | Greater than 95% as determined by SDS-PAGE. |
MW | 13 kDa |
UniProt ID | P23560 |
Protein Sequence | HSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR |
Protein Length | Full Length of Mature Protein |
Source | E.coli |
Biological Origin | Homo sapiens (Human) |
Biological Activity | The ED50 as determined by its ability to bind Human TrkB in functional ELISA is less than 5 ug/ml. |
Expression Region | 129-247aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM PB, 250 mM NaCl, pH 7.2 |
Alternative names | Brain-Derived Neurotrophic Factor; BDNF; Abrineuri Read more... |
Note | For research use only |
Application notes | Full Length of Mature Protein |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
13.5 kDa | |
Human BDNF, premium grade (orb1675341) is expressed from human 293 cells (HEK293). It contains AA His 129 - Arg 247 (Accession # P23560-1). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
Unconjugated | |
Greater than 95% as determined by reducing SDS-PAGE. | |
13 KDa | |
E. Coli |
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