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Catalog Number | orb391293 |
---|---|
Category | Proteins |
Description | Recombinant Human Pro-BDNF is produced by E.coli expression system and the target gene encoding Ala19-Arg247 is expressed. Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | BDNF |
Entrez | 627 |
UniProt ID | P23560 |
Protein Sequence | MAPMKEANIR GQGGLAYPGV RTHGTLESVN GPKAGSRGLT SLADTFEHVI EELLDEDQKV RPNEENNKDA DLYTSRVMLS SQVPLEPPLL FLLEEYKNYL DAANMSMRVR RHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a a 0.2 μm filtered solution of 20mM PB, 10% Trehalose, 100mM NaCl, 0.02% Tween 80, pH 6.0. |
Alternative names | Brain-derived neurotrophic factor; BDNF; Abrineuri Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by reducing SDS-PAGE. | |
25.6 KDa | |
Mammalian |
Unconjugated | |
95% | |
13.5 kDa | |
Human BDNF, premium grade (orb1675341) is expressed from human 293 cells (HEK293). It contains AA His 129 - Arg 247 (Accession # P23560-1). |
PLA, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human | |
Mouse | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse | |
Mouse | |
Polyclonal | |
Unconjugated |