Human BAFF-R Protein, mFc Tag

• Catalog Number: orb689403
• Category: Proteins
• Description: Recombinant Human BAFF-R protein with C-terminal mouse Fc
• Reactivity: Human
• Tag: C-Mouse Fc Tag
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• MW: The protein has a predicted molecular mass of 33.6 kDa after removal of the signal peptide.The apparent molecular mass of BAFF-R-mFc is approximately 35-55 kDa due to glycosylation.
• Target: BAFF-R
• UniProt ID: Q96RJ3
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Alternative names: BAFFR;TNFRSF13C;BAFF-R;BROMIX;CD268;CVID4;prolixin
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human IL6 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human IL6, mFc-His tagged protein (orb689393) can bind Anti-IL6 Neutralizing antibody orb689312 in a linear range of 7.81-386.8 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human IL6R, His tagged protein orb757423 can bind Human IL6, mFc-His tagged protein (orb689393) in a linear range of 0.512-40000 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human IL6R (A) were surface stained with Human IL6, mFc-His tagged protein (orb689393) 1 μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.