Human B7-2 Protein, mFc-His Tag

• Catalog Number: orb689395
• Category: Proteins
• Description: Recombinant human B7-2 protein with C-terminal mouse Fc and 6×His tag
• Reactivity: Human
• Tag: C-Mouse Fc and 6×His Tag
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• MW: The protein has a predicted molecular mass of 52.4 kDa after removal of the signal peptide.
• Target: B7-2
• UniProt ID: P42081
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Alternative names: CD86;B7-2;B70;CD28LG2;LAB72;MGC34413
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human PD-L1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human PD-L1, mFc-His tagged protein orb689384 can bind Anti-PDL1 Neutralizing antibody orb689314 in a linear range of 0.24-7.81 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human PD1, hFc-His tagged protein orb689448 can bind Human PDL1, mFc-His tagged protein (orb689384) in a linear range of 62.5-251.1 ng/ml.

Flow cytometry analysis with 15 μg/mL Human PD-L1 Protein, mFc-His tag (orb689384) on Expi293 cells transfected with human PD1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).