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Catalog Number | orb1496324 |
---|---|
Category | Proteins |
Description | Human AMIGO2, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 31 - Thr 398 (Accession # Q86SJ2-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 43.5 kDa |
Target | AMIGO2 |
UniProt ID | Q86SJ2 |
Protein Sequence | Val 31 - Thr 398 |
Source | Human AMIGO2, His Tag (orb1496324) is expressed from human 293 cells (HEK293). It contains AA Val 31 - Thr 398 (Accession # Q86SJ2-1). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Val 31 - Thr 398 |
Endotoxins | 1.0 EU per μg |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | AMGO2 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 43.5 kDa. The protein migrates as 58-62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 41.7 kDa after removal of the signal peptide. The apparent molecular mass of AMIGO2-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
≥90% as determined by SDS-PAGE | |
This protein contains the human AMIGO2(Val40-His393) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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