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Catalog Number | orb753329 |
---|---|
Category | Proteins |
Description | Recombinant human ADAM17 protein |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | N-terminal His-IF2DI Tag |
Purity | Greater than 95% by SDS-PAGE gel analyses |
MW | 29 KDa |
Formula | Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4 |
UniProt ID | P78536 |
Source | E.Coli |
Expression System | 752-824aa |
Expression Region | 752-824aa |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4 |
Alternative names | ADAM 17 protein, ADAM metallopeptidase domain 17 p Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 33.2 kDa after removal of the signal peptide. The apparent molecular mass of ADAM17(581-642)-mFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Human | |
62.5 pg/mL-4000 pg/mL | |
15.6 pg/mL |
Unconjugated | |
90% | |
63.4 kDa | |
Mouse ADAM17, His Tag (orb257936) is expressed from human 293 cells (HEK293). It contains AA Pro 18 - Asp 563 (Accession # Q9Z0F8). |
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