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Catalog Number | orb594921 |
---|---|
Category | Proteins |
Description | Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active) |
Tag | C-terminal 6xHis-tagged |
Form/Appearance | Liquid |
Purity | Greater than 95% as determined by SDS-PAGE. |
MW | 84.63 kDa |
UniProt ID | Q9BYF1 |
Protein Sequence | QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS |
Protein Length | Partial |
Source | Mammalian cell |
Biological Origin | Homo sapiens (Human) |
Biological Activity | Immobilized Human ACE-2 at 10μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-mFc .The ED50 of Recombinant 2019-nCoV S Protein RBD-mFc is 62 ng/ml . |
Expression Region | 18-740aa |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | 0.2 μm filtered 20 mM Tris, 300 mM NaCl, 1 mM ZnCl2, 10% Glycerol, pH 7.4 |
Alternative names | / Read more... |
Note | For research use only |
Application notes | Partial |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
101.9 kDa | |
SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (orb640170) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC | |
Predicted: 111.2kD Observed: 115-125kD due to glycosylation | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC | |
Predicted: 86.5kD Observed: 95-110kD due to glycosylation | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC | |
Predicted: 86.5kD Observed: 95-110kD due to glycosylation | |
HEK293 cells |
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