HSPB8 Antibody

• Catalog Number: orb1247379
• Category: Antibodies
• Description: HSPB8 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: NELPQDSQEVTCT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 20-25kDa band seen in Human Skeletal Muscle lysate [Predicted MW of approx. 22kDa according to NP_055180]. Recommended for use at 0.5-1ug/ml. Primary incubated for 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland and Tonsil. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HSPB8
• UniProt ID: Q9UJY1
• NCBI: NP_055180.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HSPB8, heat shock 22kDa protein 8, CMT2L, DHMN2, E
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 20-25kDa band seen in Human Skeletal Muscle lysate [Predicted MW of approx. 22kDa according to NP_055180]. Recommended for use at 0.5-1ug/ml. Primary incubated for 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland and Tonsil. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247379 staining (1 ug/ml) of Human Muscle lysate (RIPA buffer, 30 ug total protein per lane). Detected by chemiluminescence.

orb1247379 (10 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247379 (10 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.