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    HSPB1 (phospho-S15) antibody

    Catalog Number: orb214064

    DispatchUsually dispatched within 5-10 working days
    $ 398.00
    Catalog Numberorb214064
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to HSPB1
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Primate
    ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S15 of human HSP27 protein. The exact sequence is proprietary.
    Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetHSPB1
    Entrez3315
    UniProt IDP04792
    SourceRabbit
    StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesanti HSP27 antibody, anti HSP28 antibody, anti Hea
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    HSPB1 (phospho-S15) antibody

    Western blot analysis of HSP27 (Phospho-S15) expression in MCF7 (A), EC9706 (B) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 27 kD)

    HSPB1 (phospho-S15) antibody

    Immunohistochemical analysis of HSP27 (Phospho-S15) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    HSPB1 (phospho-S15) antibody

    Immunofluorescent analysis of HSP27 (Phospho-S15) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

    • HSPB1 (phospho-S15) antibody [orb129635]

      IHC,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      50 μl, 100 μl, 200 μl
    • HSPB1 (Phospho-S15) antibody [orb570479]

      ELISA,  IHC,  WB

      Human, Monkey

      Rabbit

      Polyclonal

      Unconjugated

      50 μg, 100 μg
    • HSP27 (phospho-S15) antibody [orb642394]

      IHC,  IP,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      25 μg, 50 μg, 100 μg, 200 μg
    • HSP27 (phospho-S15) antibody [orb644423]

      IHC,  IP,  WB

      Human

      Rabbit

      Polyclonal

      Unconjugated

      25 μg, 50 μg, 100 μg, 200 μg
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