HSPB1 Antibody

• Catalog Number: orb1253759
• Category: Antibodies
• Description: HSPB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Immunogen: HSP27 (Phospho-Ser15) antibody was raised against a peptide sequence around phosphorylation site of serine15 (G-P-S (p) -W-D) derived from Human HSP27.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 27 kDa
• Target: HSPB1
• UniProt ID: P04792
• NCBI: NP_001531.1
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using HSP27 (Phospho-Ser15).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser15). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using HSP27 (Phospho-Ser15).