HSP90 alpha/beta Antibody

• Catalog Number: orb1822490
• Category: Antibodies
• Description: Mouse Anti-Human HSP90 alpha/beta Monoclonal IgG2a
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Hyb-K41220A
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat, Saccharomyces, Yeast
• Isotype: IgG2a
• Immunogen: Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Conjugation: Unconjugated
• MW: 90kDa
• Target: HSP90 alpha/beta
• Entrez: 3326, 3320
• UniProt ID: P08238, P07900
• NCBI: NP_001017963.2, NP_031381.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.2, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: HSP86 Antibody, HSP89A Antibody, HSP90AA1 Antibody
• Note: For research use only
• Application notes: 1 µg/ml was sufficient for detection of HSP90αβ in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.

Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot analysis of Human Cell lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.