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Item 1 of 4
Item 1 of 4
HSP90 alpha/beta Antibody: Biotin
Catalog Number: orb396201
| Catalog Number | orb396201 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against HSP90 alpha/beta conjugated to Biotin. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | Hyb-K41220A |
| Tested applications | ELISA, ICC, IF, IHC, WB |
| Reactivity | Human, Mouse, Rat, Saccharomyces, Yeast |
| Isotype | IgG2a |
| Immunogen | Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304 |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000), IHC (1:100), ICC/IF (1:100) |
| Conjugation | Biotin |
| MW | 90kDa |
| Target | HSP90 alpha/beta |
| Entrez | 3326, 3320 |
| UniProt ID | P08238, P07900 |
| NCBI | NP_001017963.2, NP_031381.2 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS |
| Alternative names | HSP86 antibody, HSP89A antibody, HSP90AA1 antibody Read more... |
| Note | For research use only |
| Application notes | 1 µg/ml was sufficient for detection of HSP90αβ in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.
Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Western Blot analysis of Human Cell lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.
