HSP70 Rabbit Polyclonal Antibody

• Catalog Number: orb10848
• Category: Antibodies
• Description: HSP70 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HSP70 (481-534/641aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 70 kDa
• Target: HSPA1A
• UniProt ID: P0DMV9
• RRID: AB_10750145
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HSP70; HSP-70; HSP 70; Heat shock 70 kDa protein 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Oskoueian, Ehsan et al. Palm kernel cake extract exerts hepatoprotective activity in heat-induced oxidative stress in chicken hepatocytes BMC Complement Altern Med, 14, 368 (2014)

PubMed: 25273634

Applications

WB

Reactivity

Gallus

Yamada, Hiroshi Y. et al. Tumor-promoting/progressing role of additional chromosome instability in hepatic carcinogenesis in Sgo1 (Shugoshin 1) haploinsufficient mice Carcinogenesis, 36, 429-440 (2015)

PubMed: 25740822

Applications

IHC-P

Reactivity

Mouse

Blank control (blue line): Jurkat (blue). Primary Antibody (green line): Rabbit Anti-HSP70 antibody (orb10848), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (orb10848) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: Epididymis (Mouse) Lysate at 40 ug, Lane 3: Seminal vesicle (Mouse) Lysate at 40 ug, Lane 4: Liver (Mouse) Lysate at 40 ug, Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 6: Testis (Rat) Lysate at 40 ug, Lane 7: Spleen (Rat) Lysate at 40 ug, Lane 8: Hela (Human) Cell Lysate at 30 ug, Lane 9: Jurkat (Human) Cell Lysate at 30 ug, Lane 10: HepG2 (Human) Cell Lysate at 30 ug, Lane 11: A549 (Human) Cell Lysate at 30 ug, Lane 12: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-HSP70 (orb10848) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 68 kD.

Tissue/Cell: A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (orb10848) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.