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| Catalog Number | orb1822522 |
|---|---|
| Category | Assays and Kits |
| Description | High-Sensitivity Colorimetric detection of HSP70 |
| Reactivity | Canine, Human, Monkey, Mouse, Rat |
| Range | 0.42 - 27 ng/ml |
| Target | HSP70 |
| Sensitivity | 0.038 ng/ml |
| Storage | 4°C and -20°C |
| Alternative names | HSP70 1 ELISA Kit, HSP70 2 ELISA Kit, HSP70.1 ELIS Read more... |
| Note | For research use only |
| Application notes | 1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour while shaking at 600rpm. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at at 37°C for 1 hour while shaking at 600rpm. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at 37°C for 30 minutes while shaking at 600rpm. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm. |
| Sample Types | Plasma, Cell Lysates, Tissue |
| Expiration Date | Please enquire. |
Typical Standard Curve for the HSP70 ELISA Kit (High-Sensitivity) (Enzyme-Linked Immunosorbent Assay) Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.55 - 35 ng/mL.
