Hsp70 antibody

• Catalog Number: orb556728
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HSP70
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Human, Monkey, Mouse, Other, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HSP70 1A. The exact sequence is proprietary.
• Concentration: 0.73 mg/ml
• Dilution range: WB: 1:5000-1:20000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IP: 1:500-1:1000
• Form/Appearance: Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 70
• Target: HSP70
• UniProt ID: P0DMV9, P0DMV8
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS pH 7, 20% Glycerol
• Alternative names: heat shock protein family A (Hsp70) member 1A , he
• Note: For research use only
• Application notes: Positive control: Jurkat , Raji , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , Mouse brain , PC-12 , Rat brain , 293T
• Expiration Date: 12 months from date of receipt.

References

Shahabi, Shirin et al. Evaluating the Efficacy of Irisin Injection in Mimicking the Molecular Responses Induced by Endurance Exercise in Mouse Liver Tissue journals.lww.com, (2024)

PubMed:

Applications

IHC

Reactivity

Mouse

Immunofluorescence analysis of HeLa cells using HSP70 antibody

Immunohistochemical staining of H1299 xenograft using HSP70 antibody

Immunohistochemical staining of Rat brain using HSP70 antibody

Immunoprecipitation analysis of HeLa Whole cell lysate using HSP70 antibody

Western blot analysis of Jurkat(Lane 1), Raji(Lane 2), 293T(Lane 3), A431(Lane 4), HeLa(Lane 5), HepG2(Lane 6), H1299(Lane 7), HCT116(Lane 8), MCF-7(Lane 9), NT2D1(Lane 10), PC-3(Lane 11), U87-MG(Lane 12) using HSP70 antibody

Western blot analysis of Jurkat(Lane 1), Raji(Lane 2), 293T(Lane 3), A431(Lane 4), HeLa(Lane 5), HepG2(Lane 6), H1299(Lane 7), HCT116(Lane 8), MCF-7(Lane 9), NT2D1(Lane 10), PC-3(Lane 11), U87-MG(Lane 12) using HSP70 antibody

Western blot analysis of Jurkat(Lane 1), Raji(Lane 2), 293T(Lane 3), A431(Lane 4), HeLa(Lane 5), HepG2(Lane 6), H1299(Lane 7), HCT116(Lane 8), MCF-7(Lane 9), NT2D1(Lane 10), PC-3(Lane 11), U87-MG(Lane 12) using HSP70 antibody

Western blot analysis of Jurkat(Lane 1), Raji(Lane 2), 293T(Lane 3), A431(Lane 4), HeLa(Lane 5), HepG2(Lane 6), H1299(Lane 7), HCT116(Lane 8), MCF-7(Lane 9), NT2D1(Lane 10), PC-3(Lane 11), U87-MG(Lane 12) using HSP70 antibody